Evaluating Biological Sequences

Title of dissertation: SEARCHING, CLUSTERING AND EVALUATING BIOLOGICAL SEQUENCES Mohammadreza Ghodsi, Doctor of Philosophy, 2012 Dissertation directed by: Professor Mihai Pop Department of Computer Science The latest generation of biological sequencing technologies have made it possible to generate sequence data faster and cheaper than ever before. The growth of sequence data has been exponential, and so far, has outpaced the rate of improvement of computer speed and capacity. This rate of growth, however, makes analysis of new datasets increasingly difficult, and highlights the need for efficient, scalable and modular software tools. Fortunately most types of analysis of sequence data involve a few fundamental operations. Here we study three such problems, namely searching for local alignments between two sets of sequences, clustering sequences, and evaluating the assemblies made from sequence fragments. We present simple and efficient heuristic algorithms for these problems, as well as open source software tools which implement these algorithms. First, we present approximate seeds; a new type of seed for local alignment search. Approximate seeds are a generalization of exact seeds and spaced seeds, in that they allow for insertions and deletions within the seed. We prove that approximate seeds are completely sensitive. We also show how to efficiently find approximate seeds using a suffix array index of the sequences. Next, we present DNACLUST; a tool for clustering millions of DNA sequence fragments. Although DNACLUST has been primarily made for clustering 16S ribosomal RNA sequences, it can be used for other tasks, such as removing duplicate or near duplicate sequences from a dataset. Finally, we present a framework for comparing (two or more) assemblies built from the same set of reads. Our evaluation requires the set of reads and the assemblies only, and does not require the true genome sequence. Therefore our method can be used in de novo assembly projects, where the true genome is not known. Our score is based on probability theory, and the true genome is expected to obtain the maximum score. CLUSTERING, SEARCHING AND EVALUATING BIOLOGICAL SEQUENCE DATA